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KMID : 0894520080120010051
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Development & Reproduction
2008 Volume.12 No. 1 p.51 ~ p.56
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Effect of Culture Media on Embryonic Cell Growth in Zebrafish, Danio rerio
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Lee Ki-Young
Kim Jong-Yeon
Jo Soo-Gun
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Abstract
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To optimize the cell culture conditions of zebrafish embryonic cells, we compared the efficiency of three types of medium, DMEM, K-NAC and D-NAC. In this study, we showed that the cells grown in K-NAC have better plating efficiency than DMEM, especially in the case of low cell seeding density. However, cells grew slower in K-NAC than those in DMEM in confluent cultures. The effect of 0.1% zebrafish embryo extracts was minimal. The presence of 1% trout serum in culture medium significantly increased the growth rate of cells (p<0.05). No difference was found at 2~3×10? cell seeding density (p<0.05). At 4~5×10? cell seeding density, cells grew better in DMEM than K-NAC (p<0.1). The results suggest that supplementation of NAC and A2P in Keratinocyte SFM may improves plating efficiency when cells are plated at low population. No difference was found for cell growth in either medium with 5%, 10% or 15% FBS supplemented (p<0.05). Cells culture in D-NAC grew significantly better than those in DMEM (p<0.05). Our results clearly showed that the use of NAC and A2P in the culture medium has a positive effect on cell growth regardless of the amount of FBS added.
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KEYWORD
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Zebrafish, Embryonic cell, NAC, A2P
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